Import / Format

Generate metabarlist objects from files of various formats or directly from R objects

tabfiles_to_metabarlist()

Import 4 tables to create a metabarlist object

obifiles_to_metabarlist()

Import OBITools and associated files to create a metabarlist object

biomfiles_to_metabarlist()

Import BIOM and associated files to create a metabarlist object

read_ngsfilter()

Import an ngsfilter file

metabarlist_generator()

Create a metabarlist object

check_metabarlist()

Checks if a list of tables is a metabarlist

silva_annotator()

Including SILVAngs pipeline taxonomic annotations

silva_annotator_deprec()

Including SILVAngs pipeline taxonomic annotations

Visualise

Visualise data under both ecological and experimental perspectives

summary_metabarlist()

Metabarlist summary

ggpcrplate()

Plotting a PCR attribute in the PCR plate design

ggpcrtag()

Plotting a PCR attribute in the tag pair design

ggtaxplot()

Plotting a taxonomic tree

hill_rarefaction() gghill_rarefaction()

Generating rarefaction curves using Hill numbers

pcrslayer() pcr_within_between() FUN_pcrdist_bray_freq() FUN_pcrdist_coa_freq() check_pcr_thresh() pcr_threshold_estimate() check_pcr_repl()

Detecting PCRs replicate outliers.

Manipulate

Manipulate metabarlist objects

subset_metabarlist()

Subsetting metabarlists

extract_table()

Extract tables from a metabarlist object

aggregate_pcrs() FUN_agg_pcrs_sum() FUN_agg_pcrs_mean() FUN_agg_pcrs_prob()

Aggregate PCR replicates

aggregate_motus() FUN_agg_motus_sum()

Aggregate MOTUs

taxoparser()

Parse taxonomic information

taxodecider()

Selecting the correct taxonomy

Flag spurious signal

Detect and enable the tagging of potential molecular artifacts such as contaminants or failed PCR reactions

contaslayer()

Detecting contaminants using negative controls

tagjumpslayer()

Filtering potential tag-jumps in metabarcoding data

pcrslayer() pcr_within_between() FUN_pcrdist_bray_freq() FUN_pcrdist_coa_freq() check_pcr_thresh() pcr_threshold_estimate() check_pcr_repl()

Detecting PCRs replicate outliers.

pcr_outlier() filter_replicat() coa_function() bray_function()

Identify non-conforming PCR replicates

Export

Export data in fasta format

fasta_generator()

Generating a fasta file from a metabarlist object

Example data set

soil_euk

Soil eukaryote DNA metabarcoding data from French Guiana